| 1. Conservation Status |
| Scientific Name | Sahyadria rohani |
| Common Name | Rohan’s Denison Barb |
| Family | Cyprinidae |
| IUCN Status | Endangered (EN) |
| Distribution | Western Ghats, India — restricted to the Achankovil River system |
| Notes | Extremely limited range; threatened by habitat degradation, hydrological alteration, and collection pressure. A priority species for ex‑situ conservation. |
| 2. Natural Habitat & Ecology |
| Habitat Type | Fast‑flowing, shaded hill streams with high dissolved oxygen |
| Water | pH 6.4–7.1; GH 3–8; temperature 22–26°C; oxygen saturation > 8 mg/L |
| Substrate | Rounded stones, gravel, coarse sand, submerged roots |
| Behaviour | Rheophilic shoaling species; rapid darting locomotion; peak activity at dawn and dusk |
| 3. Water Chemistry Requirements |
| Temperature | 22–26°C (spawning optimum: 23–24°C) |
| pH | 6.4–7.1 |
| Hardness | GH 3–8; KH 2–5 |
| Flow | Strong laminar flow; turnover 8–12× per hour |
| Lighting | Moderate; prefers shaded or dappled light |
| 4. Social Structure & Behaviour |
| Group Dynamics | Best results with groups of 8–12 adults |
| Male Behaviour | Intensified red lateral stripe; rapid parallel swimming displays |
| Female Behaviour | Deeper‑bodied; increased abdominal fullness pre‑spawn |
| Territoriality | Low; brief competitive bursts during spawning |
| 5. Conditioning Protocol |
| Duration | 2–3 weeks |
| Diet | Live daphnia, mosquito larvae, bloodworms; spirulina‑rich prepared foods |
| Environmental Stability | High oxygenation; strong flow; pristine water quality |
| Indicators | Males intensify colour; females show gonadal swelling |
| 6. Spawning Strategy |
| Method | Open/substrate egg‑scatterer; no parental care |
| Spawning Sites | Coarse gravel, fine‑leaved plants, high‑flow zones |
| Spawning Behaviour | Early‑morning spawning; rapid circling; adhesive eggs scattered into substrate |
| Parental Care | None; adults must be removed immediately |
| 7. Spawning Triggers |
| Pre‑Trigger | Strong flow; high oxygen; stable temperature |
| Trigger Event | Large cool water change (20–30% at 2–4°C lower) |
| Additional Stimuli | Increased aeration; dawn light transition; elevated feeding the day before |
| 8. Eggs & Incubation |
| Clutch Size | 40–120 eggs |
| Egg Type | Adhesive; settle between gravel particles |
| Incubation | 24–36 hours at 24–26°C |
| Notes | Eggs require high oxygen and clean flow; sedimentation reduces hatch rate |
| 9. Larval Development |
| Day 0–2 | Yolk sac absorption; limited mobility; remain near substrate |
| Day 3–4 | Free‑swimming; swim bladder inflation; begin exogenous feeding |
| Day 5–14 | Require microscopic foods; rapid growth with high oxygen availability |
| Week 2–4 | Colouration begins forming; shoaling behaviour develops |
| 10. First Foods & Feeding Schedule |
| Stage 1 (Day 3–7) | Rotifers, microworms |
| Stage 2 (Day 5–14) | Small‑strain Artemia, micro‑foods |
| Stage 3 (Week 2–4) | Artemia, powdered fry diets |
| Stage 4 (4+ weeks) | Live foods; fine pellets; increased flow to strengthen musculature |